Assessment of the Immune Responses Induced in Cattle after Inoculation of a Mycobacterium bovis Strain Deleted in Two mce2 Genes

Table 1

Primers used in this study.

Primers	Sequences	Tm (°C)	Application
3′ Δmce2	F: GGATCCAAACGTTGGACCCAGACAAG		MbΔmce2
	R: GCGGCCGCGGTGCTCATTGGTTGCTACC
5′ Δmce2	F: AAGCTTTGGCGCTGCCGGTGACT		MbΔmce2
	R: GGATCCCGCGGCTACACCGTGAGATT
Mut. mce2 del	F: ACCCGAACTTCAATCTCACG		MbΔmce2
	R: ACCTCACCTATCGCATGGTC
Pol II	F: GGACCCGTGTGGACAAGAAT	76	qPCR
	R: ACAAGCCCCAGGTAATCATCC
GAPDH	F: ATCTCTGCACCTTCTGCCGA	79	qPCR
	R: GCAGGAGGCATTGCTGACA
IL-2	F: CGTGCCCAAGGTTAACGCTA	76	qPCR
	R: CCATTGAATCCTTGATCTCTCTGG
IL-4	F: TGCCCCAAAGAACACAACTG	78	qPCR
	R: GCACGTGTGGCTCCTGTAGAT
IL-10	F: GGAAGAGGTGATGCCACAGG	84	qPCR
	R: AGGGCAGAAAGCGATGACAG
IL-12p35	F: TAGCCACGAATGAGAGTTGCC	78	qPCR
	R: TTTCCAGAAGCCAGACAATGC
TNF-α	F: CCCCCAGAGGGAAGAGTCC	84	qPCR
	R: GGGCTACCGGCTTGTTACTTG
IFN-γ	F: AGCTGATTCAAATTCCGGTGG	78	qPCR
	R: GATTTTGGCGACAGGTCATTC