Research Article
Assessment of the Immune Responses Induced in Cattle after Inoculation of a Mycobacterium bovis Strain Deleted in Two mce2 Genes
Table 1
Primers used in this study.
| Primers | Sequences | Tm (°C) | Application |
| 3′ Δmce2 | F: GGATCCAAACGTTGGACCCAGACAAG | |
MbΔmce2 | R: GCGGCCGCGGTGCTCATTGGTTGCTACC | | 5′ Δmce2 | F: AAGCTTTGGCGCTGCCGGTGACT | |
MbΔmce2 | R: GGATCCCGCGGCTACACCGTGAGATT | | Mut. mce2 del | F: ACCCGAACTTCAATCTCACG | |
MbΔmce2 | R: ACCTCACCTATCGCATGGTC | | Pol II | F: GGACCCGTGTGGACAAGAAT |
76 |
qPCR | R: ACAAGCCCCAGGTAATCATCC | GAPDH | F: ATCTCTGCACCTTCTGCCGA |
79 |
qPCR | R: GCAGGAGGCATTGCTGACA | IL-2 | F: CGTGCCCAAGGTTAACGCTA |
76 |
qPCR | R: CCATTGAATCCTTGATCTCTCTGG | IL-4 | F: TGCCCCAAAGAACACAACTG |
78 |
qPCR | R: GCACGTGTGGCTCCTGTAGAT | IL-10 | F: GGAAGAGGTGATGCCACAGG |
84 |
qPCR | R: AGGGCAGAAAGCGATGACAG | IL-12p35 | F: TAGCCACGAATGAGAGTTGCC |
78 |
qPCR | R: TTTCCAGAAGCCAGACAATGC | TNF-α | F: CCCCCAGAGGGAAGAGTCC |
84 |
qPCR | R: GGGCTACCGGCTTGTTACTTG | IFN-γ | F: AGCTGATTCAAATTCCGGTGG |
78 |
qPCR | R: GATTTTGGCGACAGGTCATTC |
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