PEDF and VEGF-A Output from Human Retinal Pigment Epithelial Cells Grown on Novel Microcarriers

Figure 4

GDNF concentration time course. The GDNF concentration in the media from the hRPE cells on the different microcarriers was measured with an ELISA. The hRPE cells on Hillex II, Plastic Plus, and ProNectin F microcarriers secreted comparable amounts of GDNF at the start of the time course. The concentration was an order of magnitude less when compared to VEGF-A and PEDF. The GDNF concentration declined over the first 3 weeks, and there was no detectable amount of GDNF from day 22 on.