BioMed Research International / 2012 / Article / Fig 5

Research Article

Potential Stemness of Frozen-Thawed Testicular Biopsies without Sperm in Infertile Men Included into the In Vitro Fertilization Programme

Figure 5

Alkaline phosphatase staining of cells grown in a DMEM/F12 culture medium with added follicular fluid (passage 3, day 44). (a, b) Some attached cells with a phenotype comparable to mesenchymal stem cells and small round cells with diameters of up to 5 μm (arrow), weakly positive for alkaline phosphatase activity. (c) Negative control. (inverted microscope, Hoffman). Scale Bar: 10 μm.

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