In Vivo Clearance of Alpha-1 Acid Glycoprotein Is Influenced by the Extent of Its N-Linked Glycosylation and by Its Interaction with the Vessel Wall
Figure 1
Neuraminidase treatment of human AGP increases its clearance from the rabbit circulation. Plasma-derived AGP with (neu-AGP) or without (AGP) neuraminidase treatment was examined on a 12% SDS reducing gel, either by Coomassie Blue staining ((a), left, stained gel) or by ligand blotting with SNA ((a), right, SNA blot). The position of molecular mass markers is shown in kDa, at left. Tfn: transferrin; GST: glutathione sulfotransferase. (b) Shows the recovery (% injected dose) of acid-precipitable radioactivity in plasma at different times following injection of radioiodinated AGP (solid bar) or neu-AGP (open bar) into rabbits. *Indicates versus corresponding AGP value by unpaired -test, Welch corrected. The mean of 7 determinations ± SD is shown.
