The Potential of Cellulosic Ethanol Production from Grasses in Thailand
Table 3
Ethanol production from grasses by SSCF process.
Grasses
Ethanol
% of the theoretical values
g/L
g/g substrate
Sri Lanka
1.14 ± 0.09a
0.14 ± 0.01a
32.72 ± 2.69a
Ratchaburi
1.10 ± 0.10a
0.14 ± 0.01a
30.95 ± 2.81a,b
α-cellulose + xylan
1.06 ± 0.30a,b
0.13 ± 0.04a,b
23.01 ± 6.47b
Dwarf napier
0.98 ± 0.16a,b
0.12 ± 0.02a,b
30.60 ± 4.90a,b
Napier
0.97 ± 0.31a,b
0.12 ± 0.04a,b
30.30 ± 9.78a,b
Bana
0.87 ± 0.14a,b
0.11 ± 0.02a,b
27.28 ± 4.56a,b
Prachuap Khiri Khan
0.80 ± 0.12b
0.10 ± 0.01b
23.31 ± 3.48b
King napier
0.40 ± 0.13c
0.05 ± 0.02c
13.93 ± 4.53c
Atratum
0.36 ± 0.05c
0.05 ± 0.01c
11.64 ± 1.60c
Ruzi
0.30 ± 0.06c
0.04 ± 0.01c
9.56 ± 1.79c
Pangola
0.21 ± 0.05c
0.03 ± 0.01c
6.80 ± 1.58c
Purple guinea
0.18 ± 0.07c
0.02 ± 0.01c
6.08 ± 2.49c
Grasses (1.2 g) were pretreated with alkaline peroxide (7.5% (v/v) H2O2; pH 11.5; 35°C, 24 h). SSCF process was performed using cellulase and xylanase (72 and 1440 U, resp.) from T. reesei for hydrolysis and S. cerevisiae and P. stipitis for cofermentation at 35°C for 7 days. Positive control was 0.6 g of α-cellulose mixed with 0.6 g of xylan. Values not sharing a common superscript (a, b, c, and d) differ significantly (Duncan’s Multiple Range Test).