A New Candidate Substrate for Cell-Matrix Adhesion Study: The Acellular Human Amniotic Matrix
Figure 1
Histology of the HAM and AHAM. (a) The HAM was composed of five layers as shown by HE staining. Black arrows indicate the epithelial layer, asterisk indicates the basement membrane, and red arrows indicate the avascular stroma layer. (b) The epithelial layer and fibroblasts in the stromal side were thoroughly removed for the AHAM. Black and red arrows indicate the basement membrane and stromal layer, respectively. Scale bar, 50 m. (c) SEM image of the AHAM. The stromal layer was reticular and composed of collagen fibers with various diameters. There was no residual cell debris. Scale bar, 2 m. (d) Type I collagen, (e) type III collagen, (f) type IV collagen, (g) fibronectin, and (h) laminin were positive in immunohistochemistry staining. (i) Type VI collagen was positive in immunofluorescence staining. Scale bar, 50 m.
