Effective Silencing of Sry Gene with RNA Interference in Developing Mouse Embryos Resulted in Feminization of XY Gonad
Feminization of male gonads following knock-down of Sry. Starting at 12.5 dpc, testis cords (arrows) are visible in the control plasmid pSilencer4.1-EGFP-treated XY male gonad (a); no signs of differentiation can be observed in the control females (c) and Sry knock-down (pSilencer4.1/Sry565 treated) male gonad (b). At 13.5 dpc, clearly visible testis cords appeared in control male gonads (d), whereas in Sry knocked-down male gonad no sign of differentiation was found (e), just like the control females (f). At 15.5 dpc, testis cords can be observed clearly in control males (g), While unorganized and no visible testis cords were found in Sry knocked-down male embryos’ gonads (h), developing like the control female gonad (h). The photos were taken at transverse sections; bar = 50 μm.
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