Use of Recombination-Mediated Genetic Engineering for Construction of Rescue Human Cytomegalovirus Bacterial Artificial Chromosome Clones

Figure 2

Gene Capture Method. (a) Linearized plasmid vector (b) is amplified by primers containing homology arms to the sequences flanking the region of interest (ROI) in Toledo to create the plasmid vector cassette (c). Electrocompetent and recombination-activated DY380 harboring WT Toledo BAC (d) are used for electroporation with the plasmid vector cassette (e). Upon homologous recombination and circularization, the ROI is captured, creating the ROI rescue plasmid (f).