Use of Recombination-Mediated Genetic Engineering for Construction of Rescue Human Cytomegalovirus Bacterial Artificial Chromosome Clones

Figure 5

1% Agarose gel electrophoresis of targeted amplification reactions in WT Toledo BAC (WT) and two pUC-19-IV capture plasmids. Lane 1: 1 kb Plus DNA Ladder. Lanes 2–4: amplification of UL 132 (~850 bases) with UL 132 F and R primers. Lanes 5–7: amplification of UL 150 (~1.9 kb) with UL 150 F and R primers. Lanes 8–10: amplification of UL 147 (negative control, not within region IV) (~500 bases) with UL 147 F and R primers. Lanes 11–13: amplification of zeocin marker (positive control for capture only) (~550 bases) with Zeocin F and R primers.