Expression of GA733-Fc Fusion Protein as a Vaccine Candidate for Colorectal Cancer in Transgenic Plants
Figure 1
Schematic diagram of plant expression vectors and recombinant proteins. (a) Gene expression cassettes of GA733K, GA733-FcK, and GA733-Fc. E/35S-P, the Cauliflower mosaic virus 35S promoter with duplicated enhancer region; TEV, untranslated leader sequence of tobacco etch virus; SP, endoplasmic reticulum signal peptide; KDEL, ER retention signal; 35S-T, terminator of Cauliflower mosaic virus 35S gene. (b) Structure of recombinant proteins. (c) N-glycan structures of recombinant proteins GA733K, GA733-FcK, and GA733-Fc. The symbols of the glycan structures are as follows: N-acetylglucosamine, black square; mannose, white circle; fucose, diamond with a dot inside; xylose, white triangle.
