Expression of GA733-Fc Fusion Protein as a Vaccine Candidate for Colorectal Cancer in Transgenic Plants

Figure 6

Confocal immunofluorescence analysis of GA733K, GA733-FcK, and GA733-Fc expressed in transgenic plant cells. The Fc fragment was detected by Alexa-546 conjugated anti-human IgG (red). GA733 was detected by mouse anti-human GA733 antibody as a primary antibody and Alexa-488 conjugated anti-mouse IgG (green) as a secondary antibody. The nucleus was stained with TO-PRO-3 (blue).