Research Article
Investigation of Antimicrobial Activity and Statistical Optimization of Bacillus subtilis SPB1 Biosurfactant Production in Solid-State Fermentation
Table 2
Design of experiment and response of the central composite experimental design for the production of biosurfactant using B. subtilis SPB1.
| Run order | : temperature (°C) | : moisture (%) | : inoculum age (h) | Biosurfactant (mg/g)experimental values | Biosurfactant (mg/g) csalculated values |
| 1 | 25 | 50 | 04 | 15.22 | 15.44 | 2 | 45 | 50 | 04 | 12.47 | 13.15 | 3 | 25 | 100 | 04 | 15.40 | 15.18 | 4 | 45 | 100 | 04 | 17.40 | 17.14 | 5 | 25 | 50 | 24 | 14.90 | 15.59 | 6 | 45 | 50 | 24 | 14.20 | 14.88 | 7 | 25 | 100 | 24 | 15.85 | 15.88 | 8 | 45 | 100 | 24 | 19.10 | 19.42 | 9 | 18 | 75 | 14 | 16.20 | 15.93 | 10 | 52 | 75 | 14 | 17.40 | 16.99 | 11 | 35 | 33 | 14 | 16.58 | 15.72 | 12 | 35 | 117 | 14 | 19.50 | 19.32 | 13 | 35 | 75 | 01 | 12.50 | 12.26 | 14 | 35 | 75 | 31 | 14.50 | 14.30 | 15 | 35 | 75 | 14 | 14.30 | 14.35 | 16 | 35 | 75 | 14 | 12.80 | 14.35 | 17 | 35 | 75 | 14 | 14.91 | 14.35 | 18 | 35 | 75 | 14 | 13.20 | 14.35 | 19 | 35 | 75 | 14 | 15.80 | 14.35 | 20 | 35 | 75 | 14 | 14.49 | 14.35 | 21 | 28 | 65 | 11 | 14.42 | 14.45 | 22 | 42 | 69 | 11 | 14.74 | 14.12 | 23 | 35 | 95 | 11 | 14.48 | 15.73 | 24 | 35 | 75 | 23 | 15.95 | 14.59 |
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