Control and Augmentation of Long-Term Plasmid Transgene Expression In Vivo in Murine Muscle Tissue and Ex Vivo in Patient Mesenchymal
Tissue
Figure 3
Adenoviral vector and electroporated plasmid-mediated transgene expression in vivo. Thighs of mice were in vivo administered pCMV-luc by electroporation or AdCMV-luc (). Luminescence was analysed in vivo over time using IVIS live whole body imaging. (a) Magnitude of electroporated plasmid-mediated luciferase expression compared with Ad-mediated expression (day 18 and day 1, resp.). (b) Plasmid versus Ad transgene expression over time. Although luminescence was higher with Ad delivery, it was subject to rapid silencing to background levels by day 21, unlike electroporated plasmid.
