Heterologous Expression of Plant Cell Wall Degrading Enzymes for Effective Production of Cellulosic Biofuels

Figure 1

Outline of the major difference between the protein secretion system of Gram-positive and Gram-negative bacteria. Presence of thick outer membrane in Gram-negative bacteria demands for outer membrane permeabilization through the addition of mild detergent, lpp deletion, or by fusing the recombination protein with kill or out gene.