The Annexin A2/S100A10 System in Health and Disease: Emerging Paradigms

Figure 1

Fibrin accumulation in both 𝐴𝑛𝑥𝐴2^−/− and hyperhomocysteinemic mice. Kidney sections from 𝐴𝑛𝑥𝐴2^+/+ mice on normal chow (a and b), from 𝐴𝑛𝑥𝐴2^−/− mice on normal chow (c), or from 𝐴𝑛𝑥𝐴2^+/+ mice on a high methionine diet (d). Sections from heparin-treated, highly perfused animals were stained with a polyclonal rabbit antifibrin(ogen)-specific antibody using either immunoperoxidase plus diaminobenzidine (brown immunoreaction product) with hematoxylin counterstaining [5] (a and c), or biotinylated goat anti-rabbit IgG followed by Alexa 568-conjugated streptavidin (b and d); red staining represents Alexa 568, blue represents DAPI nuclear staining, and green is tissue autofluorescence [6].

(a)

(b)

(c)

(d)