Proteomic Profiling of the Dioxin-Degrading Bacterium Sphingomonas wittichii RW1

Table 1

Proteins identified as being increased in S. wittichii RW1 during growth on dibenzofuran when compared to growth on acetate. The spots were identified on a minimum of 6 gel images.

Master number^a	Protein accession^b	Gene locus	Protein name	Average ratio^c	𝑡-test^d
919	148556489	Swit_3587	Alkyl hydroperoxide reductase/thio-specific antioxidant/Mal allergen	1.52	0.099
922	148553900	Swit_0977	Catechol 1,2-dioxygenase	1.8	0.078
921	148555809	Swit_2901	Putative cold shock DNA-binding domain	1.89	0.078
539	148555586	Swit_2674	Adenosylhomocysteinase	1.97	0.062
418	148553385	Swit_0461	Elongation factor Ts	2.10	0.05
934	115279619	Swit_3055	Meta-cleavage pathway hydrolase	2.54	0.055
603	148555952	Swit_3046	Glyoxalase/bleomycin resistance protein/ dioxygenase	3.88	0.031

^aArbitrary identifier for spot location (see Figure 2).
^bNCBI gi∣ number.
^cUsing internal standard as 1.0, >1 is an increase and <1 is a decrease in abundance.
^dStudent’s t-test comparing spot intensity for acetate versus dibenzofuran grown cells.