Research Article
Effect of Marine Omega 3 Fatty Acids on Methylmercury-Induced Toxicity in Fish and Mammalian Cells In Vitro
| Gene | Accession number | Fwd primer (5′–3′) | Rev. primer (5′–3′) | Amplicon size | PCR efficiency |
| arp (ref. gen) | AY255630 | GAAAATCATCCAATTGCTGGATG | CTTCCCACGCAAGGACAGA | 101 | 1,813 | ef1ab (ref. gene) | BG933853 | TGCCCCTCCAGGATGTCTAC | CACGGCCCACAGGTACTG | 59 | 2,013 | fatp | CA373015/AF023258 | TGGGAGCTTGTGGGTTCAA | ACTTTCATGAGGCGGATTGG | 64 | 2,243 | actb | BG933897 | CCAAAGCCAACAGGGAGAAG | AGGGACAACACTGCCTGGAT | 92 | 2,007 | gst | BQ036247 | ATTTTGGGACGGGCTGACA | CCTGGTGCTCTGCTCCAGTT | 81 | 2,07 | tuba | BT049768 | GAGCCAGCCAATCAGATGGT | TGCGCTTGGTCTTGATTGTG | 110 | 2,009 | gpx | BQ036408 | TTCTCCACCACACTGGGATCA | GGAAATGGCATCAAGTGGAATT | 101 | 2,011 | hsp-70 | BG933934 | CCCCTGTCCCTGGCTATTG | CACCAGGCTGGTTGTCTGAGT | 121 | 1,895 | cox2 | AJ238307 | CGTCCTGAGGCAGGAGCAT | TGAGGCGTGTGGTCTGGAA | 62 | 2,18 | cpt1 | AM230810 | CTTTGGGAAGGGCCTGATC | CATGGACGCCTCGTACGTTA | 121 | 1,917 | bcl-x | NM_001141086 | GCCTGGACGCAGTGAAAGAG | GGACGGCGTGATGTGTAGCT | 107 | 2,127 |
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Full gene names: acidic ribosomal protein (arp), elongation factor 1 alpha b (ef1ab), fatty acid transport protein (fatp), β-actin (actb), glutathione-s-transferase (gst), tubulin α (tuba), glutathione peroxidase (gpx), heat shock protein 70 (hsp-70), cyclooxygenase 2 (cox2), carnitine palmitoyltransferase 1 (cpt1), and B-cell lymphoma x (bcl-x).
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