Identification of Pigment Epithelium-Derived Factor Protein Forms with Distinct Activities on Tumor Cell Lines
Deglycosylation of PEDF-1 and PEDF-2: deglycosylation of PEDF proteins was performed with PNGase F under denaturing conditions. Proteins were resolved by SDS-PAGE. (a) Western blotting of untreated and treated PEDF proteins (0.15 μg per lane) with anti-PEDF. (b) Coomassie blue staining of untreated and treated PEDF proteins (1.5 μg per lane). Migration position of PNGaseF is indicated on the right side, and molecular weight markers are on the left.