Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Figure 1
Doxycycline-regulated expression of luciferase and FTH1 in NPC S18 cells. (a) The schematic for the preparation of pTRE-Tight-BI-Luc-FTH1 used to generate the double-stable cell line, B-7, which could inducibly express luciferase and FTH1 simultaneously under the control of same promoter. (b) Luciferase activity induced by doxycycline. B-7 cells were grown in presence (Dox+) or absence (Dox−) of 10 ng/mL doxycycline for 48 h. Cells were analyzed for luciferase activity. Data were expressed as mean ± SD from 3 experiments. (c) Time-dependent FTH1 expression. B-7 cells grown in Dox containing medium were transferred to medium without Dox and grown for different durations. (d) Doxycycline dose-dependent FTH1 expression. B-7 cells were grown in medium containing doxycycline at different concentrations for 96 h. FTH1 was determined by Western blot assay. Dox, doxycycline.
