Efficiency of Ferritin as an MRI Reporter Gene in NPC Cells Is Enhanced by Iron Supplementation
Figure 2
Overexpression of FTH1 led to increase of both transferrin receptor 1 (TfR-1) and intracellular iron content. (a) Western blot assay of TfR-1 expression induced by FTH1. B-7 cells grown in the presence of doxycycline (10 ng/mL) (Dox+) were transferred to Fe containing medium (200 μM FAC) in absence of doxycycline (Dox−) followed by incubation for 72 h. (b, c) Quantification of intracellular iron content by ICP-MS. (b) B-7 cells were grown in the presence (Dox+) or absence (Dox−) of doxycycline (10 ng/mL) in the presence of FAC at different concentrations for 48 h, (c) B-7 cells grown in the presence of doxycycline at various concentrations were incubated with (FAC+) or without (FAC−) (200 μM) for 48 h. Data were expressed as mean ± SD from 3 experiments. FAC, ferric ammonium citrate. ICP-MS, inductively coupled plasma atomic emission spectrometry.
