Artificial Chromosomes to Explore and to Exploit Biosynthetic Capabilities of Actinomycetes

Figure 3

Comparison between physical maps of E. coli-Streptomyces artificial chromosomes. The vector pPAC-S2 derives from PAC, while pSTREPTOBAC V and pSBAC from BAC. The main features of the vectors are depicted: the resistance genes (neo, accIII(IV), tsr conferring resistance to kanamycin, apramycin, thiostrepton), attP-int cassettes for the site-specific integration in Streptomyces, restriction sites (BamHI, HindIII, EcoRI), sacB (conferring sensitivity to sucrose when expressed), oriT and oriV, devoted to intergeneric conjugation and inducible high copy number of the vector, respectively.