Research Article
Characterization of Porcine Endogenous Retrovirus Clones from the NIH Miniature Pig BAC Library
Table 1
Type-specific primers for screening PERV-positive clones from NIH miniature pig BAC library.
| Gene | Primer sequence (5′3′) | Annealing temperature (°C) | PCR product (bp) |
| Env-A | F: TCCGTGCTTACGGGTTTTAC | 60 | 224 | R: TTGCCAATCTTTCCATCTCC |
| Env-B | F: TAAAAGCACACCTCCCAACC | 60 | 192 | R: CCGGAATTGACAAAGGAGAA |
| Env-C | F: CACCTATACCAGCTCTGGAC | 56 | 310 | R: GTTAGAGGATGGTCCTGGTC |
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