Effect of Heat Exposure on Gene Expression of Feed Intake Regulatory Peptides in Laying Hens

Table 1

Gene-specific primers used in the analysis of gene expression.

Gene	GenBank accession no.	Primer sequences (5′-3′)	Orientation	Product size (bp)
GAPDH	NM_204305	ACATGGCATCCAAGGAGTGAG	Forward	266
		GGGGAGACAGAAGGGAACAGA	Reverse
NPY	M87294	TGCTGACTTTCGCCTTGTCG	Forward	148
		GTGATGAGGTTGATGTAGTGCC	Reverse
AgRP	NM_001031457	GGAACCGCAGGCATTGTC	Forward	163
		GTAGCAGAAGGCGTTGAAGAA	Reverse
CRH	NM_001123031	CTCCCTGGACCTGACTTTCC	Forward	86
		TGTTGCTGTGGGCTTGCT	Reverse
Ghrelin	AB075215	CCTTGGGACAGAAACTGCTC	Forward	203
		CACCAATTTCAAAAGGAACG	Reverse
POMC	NM_001031098	CGCTACGGCGGCTTCA	Forward	88
		TCTTGTAGGCGCTTTTGACGAT	Reverse
CART	BI394769	CCGCACTACGAGAAGAAG	Forward	146
		AGGCACTTGAGA AGA AAGG	Reverse
CCK	NM_001001741	CAGCAGAGCCTGACAGAACC	Forward	121
		AGAGAACCTCCCAGTGGAACC	Reverse
MCR-4	NM_001031514	ACACTCCAGCCTCTCCATTTCT	Forward	101
		TGTTCATAGCAGCCTCCCGA	Reverse
MCR-1	AY220305	CACCTACTACCGCAACAACG	Forward	218
		AGCAGATGAAGAAGACTCCCAG	Reverse
MCR-5	AB012868	TCCATTCTTCCTCCATCTCATCC	Forward	157
		CTTCCTCATTTCCTGGCTACG	Reverse