Troglitazone Induces Extracellular Matrix and Cytoskeleton Remodeling in Mouse Collecting Duct Cells

Figure 3

Troglitazone does not induce α-SMA expression in M1 cells. In the upper panel, α-smooth muscle actin (α-SMA) was assessed by Western blotting in 100 μg lysates from M1 cells treated with DMSO, confirming the absence of α-SMA, vascular smooth muscle cells (V) were used as a positive control, and 4 lanes of M1 cells treated with 10 μM troglitazone (TRO) for 24 hours. In the lower panel, α-SMA was assessed by immunofluorescence. Images are shown of M1 cells stimulated with (a) DMSO and (b) 10 μM troglitazone (TRO) for 24 hours, (c) antimouse FITC conjugated secondary as a negative control, and (d) vascular smooth muscle cells were used as a positive control. Magnification 100x.