Cigarette Smoke Extract Stimulates Rat Pulmonary Artery Smooth Muscle Cell Proliferation via PKC-PDGFB Signaling
Figure 1
(a) CSE promoted cell proliferation of rPASMCs. The rPASMCs were seeded in a 96-well plate and treated with increasing concentrations of CSE for 24 h. Cell proliferation was analyzed by MTT assay. The data are shown as mean ± standard deviation from 6 experiments and were analyzed by ANOVA. * compared with control. (b) CSE promoted cell cycle progression of rPASMCs. The rPASMCs were treated with increasing concentrations of CSE for 24 h. Cells were harvested and stained with PI solution for cell cycle assay. S phase and G2 phase cells were averaged. The data are shown as mean ± standard deviation from 6 experiments and were analyzed by ANOVA. * compared with control. (c) The PDGF signaling pathway was activated after CSE treatment. The rPASMCs were treated with increasing concentrations of CSE for 24 h. Cells were lysed and submitted to RT-PCR or immunoblot assays, with the indicated primers or antibodies.
