A Laccase with HIV-1 Reverse Transcriptase Inhibitory Activity from the Broth of Mycelial Culture of the Mushroom Lentinus tigrinus

Figure 1

Ion exchange chromatography on CM-cellulose column (2.5×20 cm). Sample: fraction of Lentinus tigrinus incubation broth that was adsorbed on DEAE-cellulose. Fraction size: 8 mL. Starting buffer: 10 mM NH₄OAc buffer (pH 4.5). Dotted line across right-hand side of chromatogram indicates linear NaCl concentration (0-1 M) gradient used to CM2 and CM3.