Patient-Derived Xenografts of Non Small Cell Lung Cancer: Resurgence of an Old Model for Investigation of Modern Concepts of Tailored Therapy and Cancer Stem Cells
Figure 1
Flow-chart of establishment of patient-derived xenografts. Fragments of primary tumor samples are initially implanted subcutaneously in both flanks of 1-2 immunocompromised mice, depending on tissue availability (). When tumors reach the exponential growth phase they are removed from donor mice, reduced into fragments, and serially transplanted in new recipient mice (). For investigational purposes (i.e., drug treatments or subpopulation analysis) PDXs can be expanded in a higher number of mice in order to obtain statistical relevant results or sufficient biological material for analysis, respectively. (: sequential passage in immunocompromised mouse; : number of mice.)
