Figure 2: hAMSCs pluripotent stem cells and ESC marker expression. Flow cytometry representative expression of the pluripotent and embryonic stem cells markers in freshly isolated hAMSCs. (a) For each staining, a gate on viable cells (red) was drawn; (b) hAMSCs were gated on the basis of morphological features; (c) cells incubated with isotypic control were used as negative controls; (d) Nanog, (e) Oct-4, and (f) SSEA4 expression in hAMSCs.