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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 579141, 9 pages
Research Article

Physical Properties and Biocompatibility of a Core-Sheath Structure Composite Scaffold for Bone Tissue Engineering In Vitro

1Institute of Orthopaedics and Traumatology, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, China
2Department of Mechanical Engineering, Tsinghua University, Beijing 100084, China

Received 1 November 2011; Revised 17 December 2011; Accepted 4 January 2012

Academic Editor: Bernd H. A. Rehm

Copyright © 2012 Chuangjian Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Scaffolds play a critical role in the practical realization of bone tissue engineering. The purpose of this study was to assess whether a core-sheath structure composite scaffold possesses admirable physical properties and biocompatibility in vitro. A novel scaffold composed of poly(lactic-co-glycolic acid)/β-tricalcium phosphate (PLGA/β-TCP) skeleton wrapped with Type I collagen via low-temperature deposition manufacturing (LDM) was prepared, and bone mesenchymal stem cells (BMSCs) were used to evaluate cell behavior on the scaffold. PLGA/β-TCP skeleton was chosen as the control group. Physical properties were evaluated by pority ratio, compressive strength, and Young’s modulus. Scanning electron microscope (SEM) was used to study morphology of cells. Hydrophilicity was evaluated by water absorption ratio. Cell proliferation was tested by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT). Osteogenic differentiation of BMSCs was evaluated by alkaline phosphates activity (ALP). The results indicated that physical properties of the novel scaffold were as good as those of the control group, hydrophilicity was observably better (𝑃<0.01) than that of control group, and abilities of proliferation and osteogenic differentiation of BMSCs on novel scaffold were significantly greater (𝑃<0.05) than those of control group, which suggests that the novel scaffold possesses preferable characteristics and have high value in bone tissue engineering.