Progesterone Downregulates Oestrogen-Induced Expression of CFTR and SLC26A6 Proteins and mRNA in Rats’ Uteri

Figure 1

Real-time PCR (a) and Western blotting analysis (b) of total homogenate of the uterus; representative photo of western blots (c) of CFTR in ovariectomized rats and rats at different stages of the oestrous cycle. Results indicate that CFTR expression is oestrogen dependent. The photos as shown in (c) came from a same gel/blot that has been cut in order to arrange the different groups of treatment in sequence as to match the result in (a) and (b). Control: peanut oil, E: 0.2 μg oestrogen, 20 E: 20 μg oestrogen, 50 E: 50 μg oestrogen, P: progesterone, E + vehicle: 0.2 μg oestrogen + peanut oil E + P: 0.2 μg oestrogen + progesterone. Ps: proestrus, Es: estrus, Ms: metestrus, Ds: diestrus: *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001.

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