Nucleolar localization of the large delta antigen of human hepatitis D virus in human hepatoma cells and in worms. Human hepatoma cells transfected with plasmids expressing GFP::LDAg, (a) visualized by GFP, (b) stained with DNA dye, and (c) the merged picture. (d–i) A transgenic worm carrying a bicistronic vector coexpressing FIB-1::GFP and RFP::LD. A low magnification of worms photographed via (d) the FITC channel to visualize FIB-1::GFP, (e) the rhodamine channel to visualize RFP::LD, and (f) the merged picture. The rectangles in panels (d–f) show four intestinal cells in the same worm. A high magnification of intestinal cells shows (g) FIB-1::GFP, (h) RFP::LD, and (i) the merged picture. Arrows indicate the nucleoli. Note that RFP::LD and FIB-1::GFP are not perfectly colocalized. Scale bars indicate 10 μm.
