Efficiency of Manual Scanning in Recovering Rare Cellular Events Identified by Fluorescence In Situ Hybridization: Simulation of the Detection of Fetal Cells in Maternal Blood
Table 2
Results obtained by manual scanning.
Slide
Spread XY cells
Detected cells
Missed cells
AMP-9
1
1
0
AMP-10
4
4
0
AMP-11
2
0
2
AMP-12
5
4
1
AMP-13
5
2
3
AMP-14
2
2
0
AMP-18
3
3
0
AMP-19
0
0
0
AMP-20
1
1
0
AMP-21
1
1
0
AMP-22
3 + 2 E*
4
1
AMP-23
0
0
0
AMP-24
1
1
0
AMP-25
1
0
1
AMP-26
3
1
2
AMP-27
0
0
0
AMP-28
1
1
0
AMP-29
0
0
0
SMP-10
4
3
1
SMP-11
11
10
1
SMP-12
2
2
0
SMP-13
11
9
2
SMP-24
5
5
0
SMP-25
3
3
0
SMP-26
2
2
0
SMP-27
3
3
0
SMP-28
1
1
0
SMP-29
5
4
1
SMP-30
6
6
0
SMP-31
3
3
0
SMP-32
6
5
1
SMP-33
7
6
1
SMP-34
6
5
1
SMP-35
4
3
1
SMP-36
2
2
0
SMP-37
1
1
0
SMP-38
3
3
0
SMP-39
2
2
0
SMP-40
5
5
0
SMP-41
4
3
1
SMP-42
0
0
0
SMP-43
3
3
0
SMP-44
1
1
0
SMP-45
0
0
0
SMP-46
1
1
0
SMP-47
0
0
0
SMP-48
2
2
0
SMP-49
0
0
0
SMP-50
1
1
0
SMP-51
1 + 1 E*
2
0
SMP-52
0
0
0
SMP-53
0
0
0
SMP-54
0
0
0
SMP-55
3
3
0
SMP-56
0
0
0
SMP-57
1
1
0
SMP-58
0
0
0
SMP-59
1
0
1
SMP-60
1
0
1
SMP-61
1
0
1
E*, extra true positive cells confirmed by re-FISH.
