Figure 1: (a) Culture of muscle-derived stem cells. MDSCs were cultured and subcultured at confluent of 80%. Bright view images were taken at day 1 and day 5 after subconfluent. (b) Immunohistochemical staining of Sca-1. MDSCs were subcultured. Expression of Sca-1 was assessed by immunohistochemical staining analysis at day 2 using an anti-Sca-1 antibody (Wuhan Boster Biological Technology Co., Ltd.).