Methodology Report
High-Resolution Whole-Mount In Situ Hybridization Using Quantum Dot Nanocrystals
Table 1
Comparative chart of different permeabilization approaches used in whole mount immunostaining using a biotinylated anti-phosphotyrosine antibody (4G10 clone) and detected with Streptavidin conjugated QDs. Proteinase K treatment is the only approach which allowed deep tissue staining using QDs.
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