Figure 1: AFSCs growth curve and doubling time. AFSCs (5,000 cells/cm2) were seeded and cultivated in 46-well plates for 12 days. At the end of each day, cells were washed, trypsinizated, and counted in hemocytometer. Each point refers to triplicate from two different AF samples. Cell doubling time calculated was 30 hours (±4 h).