Figure 1: Alterations in the expression levels identified by microarray were confirmed using qRT-PCR. Fold changes between K562 cell line treated with and without valproate detected by microarray were compared with those measured by qRT-PCR. In qRT-PCR assay, RNA was isolated from K562 cell line treated with or without valproate exclusively for validation of differentially expressed genes. mRNA levels were normalized with GADPH and fold changes were calculated by dividing mRNA level of each K563 cell line treated with valproate by mean mRNA level from control samples in triplicate. Data are mean values ± standard deviations of the means from three independent experiments performed in triplicate.