Figure 2: Cellular location of EhADH112 and the Bro1 recombinant polypeptide in transfected trophozoites. Confocal microscopy images of permeabilized ANeo, ANeoBro1, or ANeoADH112 trophozoites incubated with (a) mαEhADH112 or (b) pαFLAG antibodies. Negative controls correspond to trophozoite preparations only incubated with secondary antibodies. Top: phase contrast images. Bottom: corresponding confocal sections. Arrowheads: small cytoplasmic vesicles.