Figure 3: Effect of pre-miRNA589 on expression of miR589 in HMrSV5 cells following exposure to TGFβ1 (5 ng/ml, 24 hr). The bar graphs show the 2 Δ Δ C T value of miRNA589 relative to that of U6 in each group. The level of miRNA 589 was decreased in HMrSV5 cells treated with TGFβ1. (column 2), and this effect was abolished following transfection with pre-miR589 (column 3). *P < 0.05 versus control group, ##P < 0.01 versus TGF-β1 treated group.