Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection

Table 1

Back calculated concentrations of the calibration curves of PI polyamides in rat plasma.

HN.49
Nominal concentration (μg/mL)	0.5	1	5	10	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	0.52	0.92	4.73	10.03	21.09	51.03	102.78	194.12
Accuracy (%)	4.4	−8.4	−5.3	0.3	5.4	2.1	2.8	−2.9
Precision (%)	2.4	4.5	9.3	1.4	0.2	2.8	2.2	5.9
TGF-𝛽1f
Nominal concentration (μg/mL)	0.25	0.5	2	5	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	0.27	0.43	1.72	4.78	21.51	50.94	107.30	204.81
Accuracy (%)	7.9	−13.2	−14.2	−4.4	7.5	1.9	7.3	2.4
Precision (%)	0.8	1.2	0.1	5.6	1.8	1.3	2.0	1.0
TGF-𝛽1t
Nominal concentration (μg/mL)	0.25	0.5	2	5	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	0.26	0.47	2.01	5.31	20.39	50.71	98.07	191.09
Accuracy (%)	2.9	−6.7	0.6	6.1	1.9	1.4	−1.9	−4.5
Precision (%)	1.5	4.0	2.1	1.3	3.5	2.0	1.6	6.1
HN.50f
Nominal concentration (μg/mL)	1	2	5	10	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	1.04	1.85	5.13	9.49	20.48	51.02	103.47	196.96
Accuracy (%)	3.6	−7.5	2.7	−5.1	2.4	2.1	3.5	−1.5
Precision (%)	3.1	4.5	7.8	4.0	1.5	0.7	2.1	3.1
HN.50t
Nominal concentration (μg/mL)	0.25	0.5	2	5	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	0.26	0.47	1.89	5.06	20.53	53.40	102.25	190.47
Accuracy (%)	3.9	−6.7	−5.4	1.3	2.6	6.8	2.3	−4.8
Precision (%)	2.4	4.6	2.0	3.9	3.5	7.2	4.5	3.9
LOX-1
Nominal concentration (μg/mL)	0.25	0.5	2	5	20	50	100	200
Observed concentration; (mean of 𝑛=3, μg/mL)	0.26	0.47	1.91	5.11	20.98	51.39	101.43	190.59
Accuracy (%)	3.0	−5.3	−4.3	2.1	4.9	2.8	1.4	−4.7
Precision (%)	0.9	1.3	4.3	1.6	2.2	2.4	2.0	3.5