Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection

Table 2

Intra-day and inter-day accuracy and precision of QC samples for PI polyamides in rat plasma.

HN.49
	Intraday (𝑛=5)				Interday (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	0.5	1	100	160	0.5	1	100	160
Observed concentration; (mean, μg/mL)	0.49	0.86	104.03	144.73	0.52	0.90	103.54	154.50
Accuracy (%)	−1.2	−14.4	4.0	−9.6	3.8	−10.2	3.5	−3.4
Precision (%)	2.8	1.1	2.2	1.1	5.3	7.2	2.0	5.2
TGF-𝛽1f
	Intra-day (𝑛=5)				Inter-day (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	0.25	0.5	100	160	0.25	0.5	100	160
Observed concentration; (mean, μg/mL)	0.23	0.43	109.03	160.89	0.25	0.45	104.28	159.49
Accuracy (%)	−9.1	−13.9	9.0	0.6	1.2	−10.6	4.3	−0.3
Precision (%)	3.4	1.6	0.9	1.9	9.3	6.3	5.2	1.8
TGF-𝛽1t
	Intra-day (𝑛=5)				Inter-day (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	0.25	0.5	100	160	0.25	0.5	100	160
Observed concentration (mean, μg/mL)	0.24	0.43	101.52	147.62	0.23	0.43	100.70	149.11
Accuracy (%)	−4.7	−13.4	1.5	−7.7	−7.4	−14.4	0.7	−6.8
Precision (%)	9.5	4.7	4.5	0.4	11.9	4.1	3.9	1.9
HN.50f
	Intra-day (𝑛=5)				Inter-day (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	1	2	100	160	1	2	100	160
Observed concentration (mean, μg/mL)	1.09	1.76	109.24	172.59	1.08	1.86	107.07	171.06
Accuracy (%)	9.4	−11.9	9.2	7.9	7.5	−6.8	7.1	6.9
Precision (%)	3.3	1.2	1.7	1.8	3.9	4.2	4.2	3.6
HN.50t
	Intra-day (𝑛=5)				Inter-day (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	0.25	0.5	100	160	0.25	0.5	100	160
Observed concentration (mean, μg/mL)	0.25	0.43	97.49	143.77	0.25	0.44	97.71	149.61
Accuracy (%)	0.4	−14.2	−2.5	−10.1	0.6	−13.0	−2.3	−6.5
Precision (%)	7.7	2.6	3.6	1.8	9.0	4.5	4.4	3.4
LOX-1
	Intra-day (𝑛=5)				Inter-day (𝑛=15)
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH	LLOQ	QL	QM	QH
	0.25	0.5	100	160	0.25	0.5	100	160
Observed concentration (mean, μg/mL)	0.24	0.45	97.93	144.45	0.25	0.45	98.95	145.94
Accuracy (%)	−2.9	−10.9	−2.1	−9.7	−1.1	−10.6	−1.1	−8.8
Precision (%)	2.4	2.9	2.5	2.6	4.5	2.0	4.0	6.4