Quantitation of Pyrrole-Imidazole Polyamide in Rat Plasma by High-Performance Liquid Chromatography Coupled with UV Detection

Table 3

Recovery.

HN.49
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	0.5	1	100	160
Recovery (%)	85.6	107	91.7	93.3
Precision (%)	11.4	8.6	4.6	9.6
TGF-𝛽1f
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	0.25	0.5	100	160
Recovery (%)	76.3	101	101	98.8
Precision (%)	3.6	2.2	3.8	2.4
TGF-𝛽1t
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	0.25	0.5	100	160
Recovery (%)	101	96.5	98.2	101
Precision (%)	7.8	2.5	1.2	3.2
HN.50f
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	1	2	100	160
Recovery (%)	99.2	92.1	88.2	89.7
Precision (%)	3.0	5.3	0.8	9.4
HN.50t
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	0.25	0.5	100	160
Recovery (%)	87.2	84.4	95.6	96.7
Precision (%)	10.1	8.2	1.2	4.3
LOX-1
Nominal concentration (μg/mL)	LLOQ	QL	QM	QH
	0.25	0.5	100	160
Recovery (%)	78.3	80.6	92.7	85.8
Precision (%)	4.2	1.0	1.4	2.7