Dextran Sodium Sulphate Colitis Mouse Model: Traps and Tricks

Table 3

Profile of inflammatory mediators in different phases of DSS-induced colitis in mice.

Tissue/method	Analytes measured	acute DSS colitis	chronic DSS colitis	Mouse strain	ref.
		7 days of DSS treatment
Colon/RT PCR	IL-1, IL-4, IL-10, IL-12, IFNγ, TNFα	↑ IL-1, IL-10, IL-12,; ↑ IFN-γ, TNFα	—	BALB/c	[42]
		5 days of DSS treatment	5 days of DSS followed by 28 days of water
Colon/ELISA, MSI	IL-1α, IL-1β, IL-12 p40, IL-12 p70, IL-17, IL-6, G-CSF, IL-18	↑ IL-1α, IL-1β, IL-12 p40,; ↑ IL-12 p70, IL-17, IL-6,; ↑ G-CSF	↑ IL-1α, IL-1β, IL-12 p40,; ↑ IL-12 p70, IL-17, IL-6,; ↑ G-CSF	C57BL/6J	[22]
		↑ IL-1α, IL-1β, IL-6,; ↑ IL-18, G-CSF	↑ IL-12 p40	BALB/c	[22]
		5 days of DSS treatment	5 days of DSS followed by 7 days of water
Colon/RT PCR	TNF-α, IL-1β, IL-6, IL-10, IL-12, IFN-γ, KC, MIP-2	↑ TNF-α, IL-1β, IL-6,; ↑ IL-10, IL-12, IFN-γ,; ↑ KC, MIP-2	↑ TNF-α, IL-1β, IL-6, IL-10,; ↑ IL-12, IFN-γ, KC, MIP-2	C57BL/6J	[39]
		7 days of DSS treatment	4 cycles of 7days of DSS followed by 10 days of water
Serum/MSI	IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12, IL-17, IFN-γ, TNF-α, GM-CSF, IP-10, KC, MCP-1, MIG, MIP-1α	↑ IL-6, IL-17, TNF-α, KC	↑ IL-6, IFN-γ, IL-4, IL-10		[43]
Colon/Western	IL-6, IL-12, IL-23, IL-17, IFN-γ	↑ IL-6, IFN-γ, IL-17	↑ IL-6	C57BL/6J	[43]

RT PCR: real-time PCR; MSI: multiplex sandwich immunoassay; IFN-γ: interferone gama; IL: interleukine; GM-CSF: granulocyte-macrophage colony-stimulating factor; IP-10: interferon-inducible protein; KC: keratinocyte-derived chemokine; MCP-1: monocyte chemoattractant protein; MIG: monokine induced by IFN-γ; MIP-1α: macrophage inflammatory protein; TNF-α: tumor necrosis factor alpha.