A Novel Aspartic Protease with HIV-1 Reverse Transcriptase Inhibitory Activity from Fresh Fruiting Bodies of the Wild Mushroom Xylaria hypoxylon

Table 1

Comparion of characteristics of Xylaria hypoxylon protease with other fungal proteases.

Species	Phylum	N-terminal sequence	Chromatography behavior	Molecular mass (kDa)	pH optimum	Temperature optimum (°C)
Xylaria hypoxylon (This study)	Ascomycota	1 HYTEL LSQVV 10	Adsorbed on DEAE-, CM-cellulose, and Q-Sepharose	43	6–8	60
Aspergillus clavatus [34]	Ascomycota	1 ALTTQ SGAPW GLGSI 15	Adsorbed on CM-Sepharose	32	8.5	50
Aspergillus nidulans [35]	Ascomycota	—	—	19	5.5	65
Cordyceps sinensis [11]	Ascomycota	X DNLMR AVGAL LR X	Adsorbed on HiTrap Q XL	43	9.5	30
Helvella lacunosa [14]	Ascomycota	1 ANVVQ WPVPC 10	Adsorbed on DEAE-, CM-cellulose, and Q-Sepharose	33.5	11	65
Agaricus bisporus [9, 36]	Basidiomycota	1 MHFSL SFATL ALLVA 15	Adsorbed on DEAE-, and CM-cellulose	27	6.5–11.5	—
Armillariella mellea [10]	Basidiomycota	1 XXYNG XTXSR QTTLV 15	Adsorbed on DEAE-cellulose	55	7	55
Coprinus 7 N [37]	Basidiomycota	—	Adsorbed on DEAE-Sepharose	33	8.5	37
Grifola frondosa [38]	Basidiomycota	1 AQTNA PWGLA 10	—	20	9-10	—
Hypsizigus marmoreus [26]	Basidiomycota	1 VTQTN APWGL ARLSQ 15	Adsorbed on CM-cellulose; Unadsorbed on DEAE-cellulose	28	7.5	50
Pleurotus citrinopileatus [18]	Basidiomycota	1 VCQCN APWGL 10	Adsorbed on CM-cellulose; Unadsorbed on DEAE-cellulose and Q-Sepharose	28	10	50
Pleurotus eryngii [16]	Basidiomycota	1 GPQFP EA 7	Adsorbed on Affi-gel Blue gel and CM-Sepharose; Unadsorbed on DEAE-cellulose	11.5	5.0	45

—: no data available. Identical corresponding amino acid residues are underscored.