A Novel Aspartic Protease with HIV-1 Reverse Transcriptase Inhibitory Activity from Fresh Fruiting Bodies of the Wild Mushroom Xylaria hypoxylon

Table 2

Effects of protease inhibitors on Xylaria hypoxylon protease activity.

Protease inhibitor	Activity remaining (%)
PMSF (1.0 mM)	100.44±2.44^a
EDTA (1.0 mM)	94.23±0.84^a
Pepstatin A (0.2 mM)	63.51±0.84^b
Lima bean trypsin inhibitor (0.25 mM)	102.09±0.99^a

Activity of the control, in which inhibitors were substituted by Mes buffer (pH 7.0), was regarded as 100%. The results shown are mean ± SD (𝑛=3). Different superscripts (e.g., a, b, c) indicate a statistically significant difference (𝑃<0.05) when the data corresponding to different protease inhibitors are analyzed by analysis of variance followed by Duncan’s multiple range test.