A Novel Aspartic Protease with HIV-1 Reverse Transcriptase Inhibitory Activity from Fresh Fruiting Bodies of the Wild Mushroom Xylaria hypoxylon

Table 3

Yields and protease activities of various chromatographic fractions of Xylaria hypoxylon (from 500 g fresh fruiting bodies. Assay conditions: 37°C/15 min, 0.1 M Mes buffer, pH 7.0).

Fraction	Yield (mg)	Protease activity (u/mg)	Total activity (u)	Recovery of activity (%)	Purification fold
Extract	2038	6.2	12636	100	1
D1	506	<0. 5	—	—	—
D2	405	<0. 5	—	—	—
D3	534	20.3	10840	85.8	3.3
Q1	68	<0. 5	—	—	—
Q2	163	1.9	310	2.5	0.3
Q3	148	57.9	8569	67.8	9.3
CM1	30	<0. 5	—	—	—
CM2	31	219.4	6801	53.8	35.4
CM3	41	2.8	115	0.9	0.5
SU1	9	<0. 5	—	—	—
SU2	12	459.3	5512	43.6	74.1

—: no protease activity observed.
Protease-enriched fractions are highlighted in boldface.