Genistein Attenuates Vascular Endothelial Impairment in Ovariectomized Hyperhomocysteinemic Rats

Figure 2

Effect of genistein on vascular function in ovariectomized HHcy rats. (a) Response to PE of endothelium-intact thoracic aorta rings. 2.5% Met diet for 12 wk induced an enhanced contraction in response to PE, and ovariectomy aggravated this contraction. Supplementation with genistein for 12 wk could significantly attenuate the contraction in ovariectomized HHcy rats. ^##𝑃<0.01 versus Con; ^∗∗𝑃<0.01 versus Met, ^▴𝑃<0.05 versus OVX + Met; (b) Response to PE of endothelium-denuded thoracic aortas rings. There was no significant difference in PE-elicited contraction among groups (𝑃>0.05). (c) Response to ACh of endothelium-intact thoracic aorta rings. HHcy induced a significant decrease in relaxation to ACh, and ovariectomy further decrease this effect. Supplementation with genistein for 12 wk could significantly improve the relaxation to ACh in ovariectomized HHcy rats. ^##𝑃<0.05 versus Con; ^∗∗𝑃<0.05 versus Met, ^▴𝑃<0.05 versus OVX + Met; (d) Response to SNP of endothelium-denuded thoracic aortas rings. There was no significant difference in response to SNP among groups (𝑃>0.05). All the results were analyzed by one-way ANOVA (𝑛=6∼8).

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