Characterization of Flavonol Inhibition of DnaB Helicase: Real-Time Monitoring, Structural Modeling, and Proposed Mechanism

Figure 3

Multiple amino acids sequence alignment of DnaB helicases. Alignment was carried out using CLUSTALW2. Amino acid residues displaying 100% homology are highlighted in red, and those displaying similarity are highlighted in blue. The amino acids that are involved in ATP binding are boxed. The amino acids that are involved in ssDNA binding are shaded in gray. For clarity, only 4 bacterial stains are shown. Abbreviations: Kp, K. pneumoniae; Ec, E. coli; Gs, Geobacillus stearothermophilus [32]; Gk, Geobacillus kaustophilus [7].