Research Article
A Laccase with Antiproliferative and HIV-I Reverse Transcriptase Inhibitory Activities from the Mycorrhizal Fungus Agaricus placomyces
Table 1
Yields and laccase activities at each purification step (from 500 g A. placomyces fresh fruiting body, activity assayed at 30°C).
| | Purification step | Yield (mg) | Specific activity (U/mg) | Total activity (U) | Recovery of activity (%) | Purification fold |
| | Crude Extract | 12927.00 | 29.9 | 386468.93 | 100.00 | 1 | | DEAE-cellulose | 1150.92 | 247.61 | 284977.8 | 73.74 | 8.3 | | CM-cellulose | 184.67 | 625.86 | 115577.6 | 45.57 | 20.9 | | Q-Sepharose | 61.29 | 1187.65 | 72791.1 | 18.83 | 39.7 | | FPLC on a Superdex 75 | 42.69 | 1305.16 | 55717.3 | 14.42 | 43.7 |
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