A Laccase with Antiproliferative and HIV-I Reverse Transcriptase Inhibitory Activities from the Mycorrhizal Fungus Agaricus placomyces

Table 4

Characteristic comparison of laccases from A. bisporus [10], A. blazei [11], and A. placomyces (this study).

	A. bisporus [10]	A. blazei [11]	A. placomyces (this study)
Molecular mass (kDa)	100	66	68
Molecular structure	Monomeric	Monomeric	Monomeric
N-terminal sequence	—	—	DVIGPQAQVTLANQD
Km value	—	0.063 mM for ABTS; 1.03 mM for 2,6-DMP	0.392 mM for hydroquinone; 0.775 mM for ABTS
Optimal pH	5.6 for ρ-phenylenediamine	2.3 for ABTS; 5.5 for 2,6-DMP	6.8 for hydroquinone; 5.2 for ABTS
Optimal temperature	—	20^°C	30^°C
Antiproliferative activity towards tumor cell lines	—	—	1.8 𝜇M of MCF 7; 1.7 𝜇M of Hep G2
HIV-1 RT inhibitory activity (IC50)	—	—	1.25 𝜇M

“—”: no database.